Amylase Starch Coursework

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3, the initial step in random depolymerization is the splitting of large chains into various smaller sized segments.Currently, two major classes of alpha-amylases are commercially produced through microbial fermentation.Based on the points of attack in the glucose polymer chain, they can be classified into two categories, liquefying and saccharifying.Glucose is a simple sugar that's used as the main fuel source for the human body and most other life.Starch is a very large storage molecule composed of many glucose molecules bonded together in long chains.Starchy substances constitute the major part of the human diet for most of the people in the world, as well as many other animals.They are synthesized naturally in a variety of plants.On the other hand, the presence of alpha-1,6 glucosidic linkages results in a branched glucose polymer called amylopectin.The degree of branching in amylopectin is approximately one per twenty-five glucose units in the unbranched segments.Because the bacterial alpha-amylase to be used in this experiment randomly attacks only the alpha-1,4 bonds, it belongs to the liquefying category.The hydrolysis reaction catalyzed by this class of enzymes is usually carried out only to the extent that, for example, the starch is rendered soluble enough to allow easy removal from starch-sized fabrics in the textile industry.

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